Flip - flop of glycosylphosphatidylinositols ( GPI ’ s ) across the ER Ram
نویسندگان
چکیده
General Experimental Procedures: Egg phosphatidylcholine (ePC) and routine chemicals were obtained from Sigma Chemical Co. SM-2 Bio-beads were purchased from Bio-Rad Laboratories. ULTROL grade Triton X-100 was from Roche Molecular Biochemicals. 1-acyl-2-C6-NBD-PC (NBD-PC) was from Avanti Polar Lipids. Salt-washed endoplasmic reticulum (SWER) was prepared from a rat liver homogenate as previously described. Triton X-100, phospholipid, and protein were quantitated according to published procedures.
منابع مشابه
Flip-flop of glycosylphosphatidylinositols (GPI's) across the ER.
The transbilayer flip-flop of early intermediates in the glycosylphosphatidylinositol (GPI) biosynthetic pathway has been demonstrated using novel fluorescent GPI probes and a biochemical reconstitution approach.
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